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Figure 5


Fig. 5. Arabidopsis peroxisomes bearing overexpressed myc-AtPex11a, myc-AtPex11e or GFP-AtPex11e increase in number per cell between 5 and 45 hours post-bombardment. (A-F, upper panels) Representative myc-AtPex11-transformed cells (left side labels) were fixed at three time points (top labels) and then dual immunolabeled with anti-myc plus Cy-2-conjugated (and anti-catalase, not shown) antibodies. (G-J, lower panels) Single cells expressing chimeric proteins (left and right side labels). (G,H) GFP autofluorescent peroxisomes at 24 and 72 hours post-bombardment. (I,J) Peroxisomal colocalization of anti-CAT/Cy-2 (I) and anti-catalase/Cy-5 (J) antibodies. All panels are confocal epifluorescence projection images. Bar, 10 µm.





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