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Fig. 7. Both the C- and N-termini of AtPex11b, -c, -d and -e are on the cytosolic side of the peroxisomal membrane, whereas the N- and C-termini of AtPex11a are on the cytosolic and matrix side of the membrane, respectively. (A-T) Bombarded cells were fixed in formaldehyde at 24 (C-H) or 48 (A,B,I,J) hours, treated with pectinase, and then incubated in digitonin to permeabilize plasma (not organellar) membranes. Labels on the left of each pair of panels indicate myc-tagged proteins dual immunolabeled with anti-myc (A-U) and anti-catalase antibodies (K-V). (U-Z) Controls for digitonin permeabilization. (U-X) Anti-myc plus Cy-2-conjugated (U,W) and anti-catalase plus RhodamineX-conjugated (V,X) antibodies applied to cells transformed with myc-AtPex3p and permeabilized with digitonin (U,V) or Triton X-100 (W,X). (Y,Z) A cell from the same batch of AtPex3p-transformed cells permeabilized with digitonin and dual immunolabeled with anti-tubulin plus Cy-2-conjugated (Y) and anti-catalase plus Rhodamine-conjugated (Z) antibodies. Bars, 10 µm.

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