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Figure 2


Fig. 2. H3S10P foci are enriched in late S-phase cells and correlate with cyclin B1 expression. (A) NT2 cells submitted to a short BrdU pulse were counterstained with DAPI (grey) and labelled with BrdU (green) and H3S10P (red). Merged images illustrate the four classes of nuclei analyzed: BrdU-/H3S10P- (c), BrdU+/H3S10P- (f), BrdU+/H3S10P+ (i) and BrdU-/H3S10P+ (l). The percentage of nuclei falling into each category is indicated on the right, as well as the number of H3S10P foci (mean ± s.d.). (B) NT2 cells were counterstained with DAPI (grey) and labelled with cyclin B1 (Cyc B1, green) and H3S10P (red). Merged images illustrate the four classes of nuclei analyzed: Cyc B1-/H3S10P- (c), Cyc B1+/H3S10P- (f), Cyc B1-/H3S10P+ (i), Cyc B1+/H3S10P+ (l). The percentage of nuclei falling into each category is indicated on the right. Bars, 10 µm.





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