JCS03317 Supplementary Material

Files in this Data Supplement:

• Supplemental Table 1 - Adobe PDF

• Supplemental Table 2 - Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Inhibition of sumoylation by Gam1 perturbs cell morphology of stably differentiated HaCaT cultures, but not of basal HaCaT cultures. HaCaT cultures were maintained in low (BASAL) or high calcium (DIFFERENTIATED) medium for at least 3 weeks prior to infection with Ad-Gam1 or Ad-GFP as indicated. Infections were at an MOI of 300 for each virus. The DIFFERENTIATED HaCaT culture infected with Ad-GFP was also treated with 100 mM TSA as in Fig. 7. Phase-contrast (black and white) and fluorescence microscopy (green) images were taken every 24 hours for 3 days.