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Figure 1


Fig. 1. HaCaT cell cultures exhibit phenotypic differences in response to Ca2+ concentration. (A) Phase-contrast microscopy of HaCaT cells maintained in low [Ca2+] medium (0.03 mM Ca2+) or in high [Ca2+] medium (2.8 mM Ca2+). Upon Ca2+ addition to the medium, basal HaCaT cells take 4-7 days to assume a complete differentiated state. Inversely, upon Ca2+ depletion, differentiated HaCaT cells take about 3 weeks to revert to a basal phenotype. (B) RT-PCR analyses of total mRNA harvested from basal (0.03 mM Ca2+) and differentiated HaCaT (2.8 mM Ca2+) cultures. (C) Immunoblot analyses showing expression of keratin 1, involucrin, and {alpha}-tubulin in total cell extracts harvested from basal (0.03 mM Ca2+) and differentiated (2.8 mM Ca2+) HaCaT cultures.





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