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Figure 6


Fig. 6. The pattern of SUMO-conjugated substrates changes during HaCaT cell differentiation. Total cell extracts were prepared at the indicated times post Ca2+ induction and analyzed by immunoblotting using antibodies to SUMO1 (upper panel), {alpha}-tubulin (middle panel) or keratin 1 (lower panel). Protein concentrations in the samples were equalized based on the {alpha}-tubulin levels. The positions of molecular size markers are shown on the right. Arrows and arrowheads indicate bands whose quantity increased or decreased, respectively, during the time period examined.





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