QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 7. Inhibition of sumoylation perturbs normal HaCaT cell differentiation. (A) HaCaT cells undergoing Ca²⁺-induced differentiation exhibit abnormal morphology when expressing Gam1 protein. Shown are phase-contrast and fluorescence microscopy images of HaCaT cell cultures at 24-hour intervals. HaCaT cells were infected with Ad-Gam1/Ad-GFP (upper panel; MOI 150/150) or Ad-GFP alone (middle and bottom panels; MOI 300) and induced to differentiate by switching to high [Ca²⁺] medium. For the bottom panel, 100 mM TSA (an HDAC inhibitor) was added 24 hours post infection and induction and was re-added in fresh medium at 48-hour intervals. (B) Immunoblot analysis for Ubc9, Gam1, and -tubulin in infected HaCaT cells. Cells were infected with the Ad-Gam1 (MOI 300), Ad-Gam1/Ad-GFP (MOI 150/150), or Ad-GFP (MOI 300) and induced to differentiate as in A. Cell extracts were prepared at the indicated times and immunoblotted. (C) Immunoblot analysis for keratin 1 and -tubulin. Cells were infected with the indicated adenovirus constructs at an MOI of 300. Cell extracts were prepared and analyzed as in B.

Return to article