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Figure 1


Fig. 1. Paxillin is tyrosine phosphorylated in focal complexes (FXs) and focal adhesions (FAs), but not in fibrillar adhesions (FBs). (A) Porcine aortic endothelial cells (PAECs) were fixed and double-stained for paxillin and phospho (Y118) paxillin. Arrows indicate an FA and arrowheads an FB. Note the high level of phosphorylated paxillin in FXs and FAs, and its absence in FBs. The intensity profile of paxillin (blue line) or phosphorylated paxillin (pink line) along a line one-pixel wide, spanning both an FA and an FB, is shown. (B) PAEC staining showing the co-localization of paxillin and tensin in both FA and FB. Bars, 10 µm (A); 5 µm (B).





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