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Figure 3


Fig. 3. Phosphomimetic and non-phosphorylatable paxillin mutants affect the adhesive phenotype of endothelial cells. Porcine aortic endothelial cells were transfected with phosphomimetic (Y2E) or non-phosphorylatable (Y2F) mutants of YFP-paxillin and with wild-type YFP-paxillin as a control. To verify that the lack of FB in Y2E cells and the lack of FX in Y2F cells was genuine, the transfected cells were stained for tensin (blue) and general phosphotyrosine (PY, red). Bar, 5 µm.





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