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Figure 4


Fig. 4. Phosphomimetic paxillin enhances adhesion dynamics and non-phosphorylatable paxillin stabilizes adhesion sites. Time-lapse movies of endothelial cells, expressing wild-type YFP-paxillin or phosphomimetic (Y2E) or non-phosphorylatable (Y2F) mutants, were used to create temporal ratio images (A) and for autocorrelation analysis (B). For each construct, the ratio between two time points, 10 minutes apart, is presented in color code, such that new pixels are red, pixels that disappeared are blue and unchanged pixels are yellow. Note the high proportion of red and blue pixels in Y2E-paxillin and the predominance of yellow pixels in Y2F-paxillin. Inserts are enlarged three times. For each construct, seven movies were used to perform autocorrelation analysis, either on the whole cell or specifically for FA/FB. Autocorrelation is calculated by comparing the adhesions in each time point with time zero. All points are plotted, as well as smoothing spline fits ({lambda}=1000) as specified to the right. Bar, 5 µm.





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