spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Inhibition of osteoclast differentiation from BMMs and PBMCs by HA. (A,B) Effect of HA on osteoclastogenesis from BMMs. Mouse primary BMMs were cultured for 6 days with RANKL (100 ng/ml), M-CSF (30 ng/ml) and indicated concentrations of HA. After TRAP staining, TRAP+ multinuclear cells (TRAP+ MNCs) with more than three nuclei were scored as osteoclasts. *P<0.05 and **P<0.01 compared with vehicle-treated control. (C) No effects of HA on osteoclastic differentiation of RAW264.7 cells. Cells were cultured for 4 days with RANKL (100 ng/ml) and HA (0.1-5 µg/ml) and stained for TRAP, and TRAP+ MNCs were counted. (D) Effect of HA on human PBMC differentiation to osteoclasts. Human PBMCs were cultured for 9 days with RANKL (200 ng/ml), M-CSF (30 ng/ml) and indicated concentrations of HA. Cells were stained for TRAP and TRAP+ MNCs were counted. *P<0.05 and **P<0.01 compared with vehicle-treated control.





Right arrow Return to article