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Figure 3


Fig. 3. Activation of Rap1 in endothelial cells induces cell-cell junction maturation. Anti-VE-cadherin and F-actin staining of HUVE cells treated for 30 minutes with vehicle or the Epac-specific cAMP analog 007. Stimulation with 007 clearly increased cell-cell junction maturation: the adherens junctions form a smoother line between the cells. Endothelial cell permeability is reduced as a biological consequence of the junction tightening (Bar, 10 µm).





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