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Fig. 6. Transformation of Paramecium cells with the GFP-act8-1 construct resulted in a weak cytosolic signal, labeling around some food vacuoles (fv; A,C) and at the presumed peniculus (p) and the quadrulus (q) of the oral cavity (A,B; C, detail from A). From the latter, a dynamic fiber system originates (B, see also supplementary material Movie 2). Close to the surface round to elongate structures are labeled (arrow in B) which continue deeper into the cytoplasm and probably represent Golgi fields (see text). The superficial plane in D reveals regularly spaced, labeled, very small dots at the plasma membrane (small bar between two labeled structures measures 2 µm; double arrow represents direction of cell axis), i.e. the sites occupied by `parasomal sacs'. (E,F) In immunogold EM analyses, using anti-GFP gold-antibody, label was enriched at the boundary of the rough ER in association with smooth vesicular, round to elongate membrane-bounded elements (arrowhead in E) or in bona fide Golgi fields (F). mn, macronucleus; oc, oral cavity; cv, contractile vacuole. Bars, 10 µm (A-D); 100 nm (E,F).

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