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Figure 9


Fig. 9. Comparison of the phagocytotic capacity (as defined in Materials and Methods) of cells silenced in different actin subfamilies. Although silencing of members of the act1 subfamily, act5 or act6 does not significantly affect phagocytosis (P<0.05; control: pPD vector), it is reduced to ~70% in cells silenced in act7, and to ~30% in cells silenced in act2 or act3. With act9, in normally shaped cells phagocytosis is slightly impaired, whereas there is a strong inhibition in triangle-shaped cells (~10% phagocytotic activity). In cells silenced in act4, no phagocytosis could be observed (*). Data were collected from five different experiments from at least 20 cells per group. Error bars represent s.e.m.





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