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Figure 3


Fig. 3. Overexpression of Fgr, but not Lck, leads to mis-sorting of FasL. (A-F,J,K) Confocal images of transiently transfected RBL expressing FasL-GFP (A,B), Fgr-GFP (C-E), Fgr-YFP (F) and Lck-GFP (J,K) or untagged FasL (E,K) and stained with antibodies against Lgp120 (A,C), EEA1 (B,D,F,J) or FasL (E,K). Scale bars represent 10 µm (C,D,E,J,K) or 25 µm (A,B,F). (G-I) FACS plots showing cell surface expression of FasL (G,I) or CD63 in RBL expressing equal levels of FasL-GFP or CD63-GFP. Surface staining was measured using antibodies against the extracellular domains of FasL, or CD63 in the presence of the metalloprotease inhibitor BB3013. RBL expressing FasL-GFP alone are shown as red histograms and RBL expressing FasL-GFP and either Fgr (G,H) or Lck (J) are shown as blue histograms.





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