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Figure 7


Fig. 7. Phosphorylation and ubiquitylation modifications are required for FasL sorting into MVBs. (A) Immunogold labelling of cryo-sections from stable transfectants of RBL expressing equivalent levels of WT, {Delta}-Pro, KKR and 3Y GFP-tagged constructs of FasL, detected with NOK-1 labelling of the extracellular domain of FasL and Protein A-gold. Bars, 100 nm. (B) Quantification of number of gold particles in MVBs when WT, {Delta}-Pro, KKR and 3Y FasL are expressed in RBL. Equivalent expression levels of FasL were confirmed by western blotting.





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