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Fig. 2. Subnuclear distribution of hOGG1-GFP in UVA-irradiated cells. (A) HeLa cells expressing hOGG1-GFP were UVA irradiated and incubated for 2 hours at 37°C. After fixation, DNA was stained with PI-RNase (red). (B) UVA-irradiated cells were immunostained with anti-B23 antibody (red). (C) UVA-irradiated and NI cells were immunostained with anti-SC35 (red), arrows indicate the absence of hOGG1-GFP in nuclear speckles in NI cells. The positions of the line scans shown in D are indicated in the merged images. (D) CCF analysis and line scans of hOGG1-GFP (green) and SC35 (red) signals. In UVA-irradiated cells a maximum overlap coefficient and a peak around X=0 indicates a positively correlated, nonrandom colocalisation. In the line scan, the fluorescence intensity peaks for hOGG1-GFP and SC35 perfectly coincide, indicating the colocalisation of hOGG1-GFP with nuclear speckles. Conversely, in the NI cells, the CCF profile indicated an exclusion of hOGG1-GFP from nuclear speckles. Bars, 4 µm (A,B); 2 µm (C).

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