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Figure 5


Fig. 5. Oxidative stress induced by UVA triggers hOGG1-GFP recruitment to nuclear speckles. (A) Visualisation of ROS production in NI and UVA-irradiated cells by CH2-DCFDA green fluorescence. DNA was stained with DAPI. (B) Total, oxidised (GSSG) and reduced (GSH) glutathione levels were measured in NI and UVA-irradiated cells. Bars represent the average of two experiments ± s.d. (C) Addition of 2 mM of NAC during UVA irradiation and recovery periods prevents the formation of hOGG1 foci. DNA was stained with DAPI. Bars, 4 µm.





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