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Figure 1


Fig. 1. Isolation of glycodelin-A binding human sperm surface proteins. (A) Silver staining of 12% SDS-PAGE gel. (B) Detection of biotinylated protein using streptavidin-alkaline phosphatase conjugate. (C) Detection of FUT5 using the polyclonal anti-FUT5 antibody, N-18. Lane 1: sulfo-SBED-conjugated glycodelin-A in non-reducing conditions. Lane 2: sulfo-SBED-conjugated glycodelin-A in reducing conditions. Lane 3: immunoaffinity-purified cross-linked glycodelin-sperm protein complex in non-reducing conditions. Lane 4: immunoaffinity-purified cross-linked glycodelin-sperm protein complex in reducing conditions. Lane 5: similar to lane 4 with the use of human fibroblasts instead of spermatozoa. Lane 6: similar to lane 5 with the use of human fibroblasts instead of spermatozoa. Lane 7: molecular mass marker.





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