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Fig. 2. Western blot analysis of sperm protein extract probed with anti-fucosyltransferase antibodies (1:200). (A) D-17 against the C-terminus of FUT5. N-16(B) N-18 (C) and G16 (D) against the N-terminus of FUT3/FUT6, FUT5 and FUT6, respectively. Lane 1: molecular mass marker. Lane 2: 10 µg sperm protein extracts. Lanes 3-5: 5 µg of recombinant FUT3, FUT5 and FUT6, respectively.