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Figure 8


Fig. 8. SNX6 co-immunoprecipitates with endogenous SNX1. (A) HeLa cells were transiently transfected with Flag-tagged constructs encoding either full-length SNX5 or SNX6. After 48 hours incubation, cell lysates were incubated with anti-Flag antibodies as described in Materials and Methods. The resultant immunoprecipitates were resolved by SDS-PAGE prior to western blotting with anti-SNX1 antibodies. Whereas endogenous SNX1 strongly co-precipitates with overexpressed SNX6, only an insignificant amount is co-precipitated with Flag-SNX5 (B,C) Endogenous SNX5 or SNX6 were immunoprecipitated and probed for co-immunoprecipitation with SNX1. A fraction of endogenous SNX1 is stably associated with SNX6. IgG HC, heavy chain of mouse IgG antibody; P, pellet; S, supernatant.





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