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Figure 2


Fig. 2. SB-431542 inhibited ES cell propagation. (A) Colony morphologies of MGZ5 cells treated with SB-431542. MGZ5 cells were seeded at 2000 cells/well in six-well plates and cultured in FCS-containing medium supplemented with 10 µM SB-431542 (right) or DMSO (as control, left) for 1 week. Numbers indicate numbers of colonies appearing (n=3). Each lower panel shows AP staining. Bars, 5 mm (upper panels); 200 µm (lower panels). (B) Relative numbers of cells treated with SB-431542 or DMSO present on Day 7 of culture compared with that at Day 0. Each bar represents the mean ± s.e.m. (n=3). (C) Relative proliferation intensity is shown as total cell number/number of colonies appearing, compared with that of cells treated with DMSO (100%). Each bar represents the mean ± s.e.m. (n=3). (D) Luciferase reporter assay of MGZ5 cells transfected with reporter constructs (ARE-luc reporter or Id-1-luc reporter) and treated with SB-431542 or DMSO (as a control). Each bar represents mean ± s.e.m. (n=3).





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