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Figure 3


Fig. 3. The Ce-lamin tail domain binds to mitotic chromosomes and the NLS motif is important for this binding. Binding of full-length Ce-lamin, Ce-lamin tail domain and tail domain mutated in its NLS (mNLS) to mitotic CHO chromosomes was performed in the presence of 10% BSA. The control did not include the Ce-lamin protein. Chromosomes were co-stained with DAPI (left) and with affinity purified polyclonal Ce-lamin antibody as primary antibody and Cy3-conjugated anti-rabbit antibodies as secondary antibodies (right). Quantification of the fluorescent signal (far right) was performed using the Science Laboratory 99 Image Gauge software and is given as the average fluorescence signal of binding to mitotic chromosomes in arbitrary units/sq. pixel. Data was taken from at least three independent experiments. SD, standard deviation.





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