QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. Blot overlay assay showing that lamin Dm₀ binding to histone H2A requires both the N- and C-tail domains of histone H2A. (A) Schematic view of the different histone H2A-derived polypeptides used in the blot overlay and chromosome binding experiments. (B,D,E) Equal amounts of protein (6 µg) were resolved using SDS-PAGE and transferred to a nitrocellulose membrane. Relative protein content was confirmed by staining the membrane with Ponceau S before incubation with the overlay solution (Ponceau, top panels). Immunostaining was performed with 611A3A6 monoclonal lamin Dm₀ antibody after incubation with overlay buffer containing 12 µg/ml lamin Dm₀ tail domain protein (ECL, bottom panels). Purified proteins and their corresponding overlay signals are indicated above the lanes. (C) Increasing amounts of the synthetic H2A N-tail peptide (N-tail) were used in the blot overlay experiments. BSA was used as a negative control (shown only in C).

Return to article