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Figure 1


Fig. 1. Effects of cytochalasin D on cell-cell adhesion. MDCK cell layers were treated with DMSO or 10 µM cytochalasin D for the indicated times and then cytochalasin D was washed out, and cells were incubated in fresh medium for the indicated times. (A) Cell layers were fixed and stained for WAVE2, ß-catenin and actin filaments (F-actin). Bar, 10 µm. (B) Vertical sections. Bar, 5 µm. (C) The signal intensities for ß-catenin and WAVE2 of cross sections as shown in A were quantified with ImageJ software. All data are mean ± s.d. *P<0.05; **P<0.0005. Statistical significance was examined using the Student's t-test.





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