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Figure 7


Fig. 7. WAVE2 functions downstream of Rac cell-cell contact sites. (A) DECMA-1 inhibits the RacCA-induced development of cell-cell adhesions. Cells expressing Myc-tagged RacCA were trypsinized and then re-plated in media containing DECMA-1. Cells were fixed and stained for Myc, WAVE2, and actin filaments. Arrows indicate the areas magnified the right-hand panels. Bars, 15 µm. (B) Effects of WAVE2 RNAi on RacCA-induced development of cell-cell adhesion. Myc-tagged RacCA was transiently expressed in CTRL siRNA and WAVE2 siRNA-treated cells, and its effects on cell-cell adhesions were examined. Cells were fixed and stained for ß-catenin and actin filaments. Arrowheads and arrows indicate the cell-cell contacts in Myc-tagged RacCA expressing or non-expressing cells, respectively. Bar, 7.5 µm. (C) Signal intensities of ß-catenin and actin filaments were quantified in vertical sections with ImageJ software. All data are mean ± s.d. *P<0.05; **P<0.005; ***P<0.0001. Statistical significance was examined using the Student's t-test.





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