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Fig. 8. Rac signaling is important for WAVE2 localization at cell-cell contacts. (A) Myc-tagged constitutively active (CA) and dominant-negative (DN) Rac were transiently expressed, and the localization of WAVE2 was examined by immunostaining. The regions from which sections were taken are indicated by white lines. Arrows indicate the positions of cell-cell contacts. Bar, 7.5 µm. (B) In MDCK cells transiently expressing GFP-Rac, the Rac-GFP formed clusters at sites other than cell-cell contacts (arrows). These clusters contained E-cadherin, actin filaments, and WAVE2. Bar, 15 µm. (C) CTRL or Tiam1 siRNA-treated cells were fixed and stained for WAVE2, E-cadherin, and actin filaments (F-actin). The regions from which vertical sections were taken are indicated by white lines. Arrows indicate localization of WAVE2. Bar, 10 µm.
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