spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 9


Fig. 9. WAVE2 is recruited to cell-cell contacts through its N-terminus. (A) GFP-tagged WAVE2 constructs were expressed and their localization was examined and quantified in MDCK cells. All data are mean ± s.d. Three independent experiments were performed. WHD, WAVE-homology domain; B, basic domain; PR, proline-rich domain; VCA, verprolin-homology, cofilin-homology and acidic domain. Bar, 15 µm. (B) Pull-down assay. (Left panels) MDCK cell lysates were incubated with GST-tagged truncated WAVE2 proteins and immunoblotted with anti-Abi1 and anti-HSPC300 antibodies. (Right panels) MDCK cells in which GFP-tagged Abi1 or HA-tagged HSPC300 were expressed were lysed and then incubated with GST-tagged full-length WAVE2 proteins and immunoblotted with anti-GFP and anti-HA antibodies, respectively. (C) WHD-GFP was transiently expressed in CTRL, Abi1 or HSPC300 siRNA-treated cells. Expression levels of WHD-GFP were similar in these cells (data not shown). Cells were fixed and the localization of WHD-GFP was examined and quantified. These data indicate that localization of WHD was dependent on Abi1 and HSPC300. Bar, 30 µm. All data are mean ± s.d. of three independent experiments.





Right arrow Return to article