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Files in this Data Supplement:
Movie 1. DIC time-lapse recording of mitosis in a vertebrate cell (PtK1). The movie begins in late prophase when chromosomes are already condensed inside the nucleus and the nucleolus has largely disappeared. Nuclear envelope breaks down at 14:00 (time in minutes: seconds) and chromosomes immediately begin to move in the cytoplasm. Note that many chromosomes migrate to one of the two spindle poles and become ‘mono-oriented’ before they congress to the spindle equator. Typical examples of congression can be seen between 54:00 and 1:08:00. Approximately 10 minutes after congression of the last mono-oriented chromosome (1:12:00) the cell initiates anaphase (1:22:00). Selected frames from this movie are shown in Fig. 2.
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