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Figure 2


Fig. 2. EBs formed from PDK1-/- ES cells are reduced in size and cellularity. EBs were generated from wild-type parental (WT) and PDK1-/- ES cells. (A) Examples of day 5 EBs, original magnification 100x (10x/0.4 objective lens was used). Bars, 200 µm. (B) On day 3 to day 6, samples of EBs were trypsinised and the cells counted. Data shown are the mean (± s.e.m.) from two to three independent experiments. (C) The mean number of EBs formed, counted on day 6 (± s.e.m.) from three independent experiments. (D) Protein extracts from EBs formed from wild-type (WT) or PDK1-/- (ko) ES cells were prepared on the days indicated, separated by SDS-PAGE and immunoblotted with antibody against phosphorylated S6 ({alpha}-P-S6). The blot was then stripped and reprobed for GAPDH. *P<0.05, ***P<0.005.





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