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Fig. 1. ASE-triggered Ca²⁺ oscillations stop in the absence of pronuclei. (A) A series of images showing the procedure by which eggs were separated into small and large cytoplasts. See Materials and Methods for details. Bar, 50 µm. (B) Pseudocolour images showing that the Ca²⁺ oscillations progress as waves through the cytoplasm of both large and small cytoplasts. Low Ca²⁺ is indicated in magenta, higher levels in blue and highest by yellow, 10-second time lapse between images in upper panel, 5 seconds in the lower panel. (Ca) The ASE-induced Ca²⁺ oscillations stop in both cytoplasts simultaneously. Each cytoplast was injected with ASE within 30 seconds of each other. The traces from both cytoplasts show the oscillations stop in the smaller (blue line) at approximately the same time as the larger (red line) which extruded two polar bodies, evidence that this cytoplast contained the chromatin. (b) Mean time (± s.e.m.) at which the oscillations stop in cytoplasts with (red bar) and without (blue bar) chromatin (P=0.5, n=10, nine animals).

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