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Figure 6


Fig. 6. Coronin 1B can bundle actin filaments in vitro. (A) Electron micrograph of actin filaments, inset showing 4x magnification. Actin filaments (1 µM) were negatively stained and examined by transmission electron microscopy. Arrowheads indicate areas where filaments lie with close apposition; bar, 100 nm. (B) Electron micrograph of actin filaments (1 µM) with coronin 1B (10 nM); bar, 100 nm. (C,D) Electron micrograph of actin filaments (1 µM) with either wild-type coronin 1B or the R30D mutant (1 µM); bar, 1 µm. (E) Representative Coomassie-Blue-stained gel showing low-speed co-sedimentation of coronin 1B with F-actin. Samples were prepared as described in C and D, then subjected to centrifugation for 5 minutes at 13,000 g.





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