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Figure 2


Fig. 2. TERT-expressing cells in adult heart are associated with Sca-1 expression. (A) Telomerase activity was measured by the TRAP assay in three independent clonal-CSCs. The cells were treated with or without heat and used as templates. Hela cells were used as positive controls. (B) Construction of EGFP transgene under the control of the TERT promoter. (C) PCR of genomic DNA from 2 independent TERT-EGFP transgenic lines and respective NTG littermate controls. (D) The expression of TERT on EGFP-positive and EGFP-negative cells sorted from TERT-EGFP transgenic hearts is shown by RT-PCR. The TERT expression was detectable in all three independent clonal CSCs shown in Fig. 1C. (E) FACS analysis of the primary EGFP-positive cells isolated from TERT-EGFP mice (TG). Expression of Sca-1, KIT, CD45, CD34, and CD31 in EGFP-positive cells was examined. Cells from NTG littermates were used as negative control. Data are representative of six independent experiments.





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