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Figure 5


Fig. 5. Loss of Sca-1 transcripts does not affect the differentiation potential of clonal CSCs. (A) Differentiation of NTG-derived or Sca-1 KD-derived clonal CSCs. Cardiac muscle cell (cardiac troponin-T), endothelial cell (CD31) and vascular smooth muscle cell ({alpha}-SMA) differentiation were 1.24±0.3%, 12.4±1.8% and 31.9±2.5%, respectively, for NTG-CSCs (C2, C3 and C6, respectively), and 1.23±0.3%, 12.1±2.1% and 32.2±4.7%, respectively, for Sca-1 KD-CSCs (C1). Nuclei are stained by DAPI (blue). Bars, 20 µm in A. (B) RT-PCR showed that the differentiation potential into the three different lineages were similar for both types of CSCs (n=3). (C) Representative Ca2+ transient in beating cardiomyocytes. Clone 2, 3 and 6 from NTG and clone 1 from Sca-1 KD mice, all expressed brachyury at baseline, were used for analysis. Intensities were corrected by background amplitude and expressed as arbitrary units (n=3).





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