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Figure 8


Fig. 8. Loss of Sca-1 transcripts in clonal CSC transplantation shows less donor-cell engraftment, resulting in the decrease in late cardiovascular regeneration. (A) Engrafted lacZ+ cells in NTG (C6)- and Sca-1 KD-CSC (C1)-transplanted hearts at day 7 after infarction. Sections were counterstained using H&E. (B-D) The representative images and frequencies of cardiomyocytes (cardiac troponin-T, red), and endothelial (CD31) and smooth muscle cells ({alpha}-SMA) in lacZ+ cells at day 28 are shown (n=6). Note that differentiated lacZ+ cardiomyocytes co-express connexin-43 (yellow). Bars, 100 µm in A; 20 µm in B; 50 µm in C,D.





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