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Fig. 2. Force enhances phosphorylation of LIMK1 and cofilin. (A,B) Immunoblots of LIMK1 and phosphorylated LIMK (p-LIMK) in lysates of Rat-2 cells. The ratio of phosphorylated to total LIMK was computed from densitometry measurements of immunoblots. The data are mean ± s.d. compared with the no-force ratio (designated as 1). For cells treated or not with Y27632, the difference between force and no force at 5 minutes (A) or 15 minutes (B) is statistically significant (P<0.05). (C,D) Immunoblots of cofilin and phosphorylated cofilin (p-cofilin) in Rat-2 cells as measured for LIMK1.
