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Figure 1


Fig. 1. VEGF and FGF2 have additive effects on the induction of capillary formation by BREC in a 3D collagen assay. (A) Phase-contrast images of BREC after 4 days in collagen, untreated or treated with 25 ng/ml VEGF, or with 25 ng/ml VEGF plus 2.5 ng/ml FGF2. (B) Total length of CLS after 2 (white bars) and 4 (black bars) days. Treatment with VEGF or VEGF plus FGF2 led to a significant increase in the total length of CLS at both 2 and 4 days (P<0.0006 in all comparisons). Administration of VEGF plus FGF2 for 4 days induced the longest CLS and was significantly different from VEGF (4 days, *P<0.03) and VEGF plus FGF2 (2 days, **P<0.007). Proliferation was assessed on the third day, after exposure to the growth factors for 13 hours (incorporation of BrdU over 12 hours). VEGF plus FGF2 caused a significant increase in the number of BrdU-positive cells vs no treatment or VEGF only (#P<0.0009). (C) Visualization of endothelial nuclei, apoptotic ECs and proliferating ECs on the third day, after treatment with VEGF plus FGF2 and application of BrdU for 12 hours. Bar, 100 µm.





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