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Figure 5


Fig. 5. EGF induces trafficking of a pool of E-cadherin to the late endosome. MCF-7 cells were stimulated with EGF (30 minutes), fixed and immunolabeled for E-cadherin (green) and nuclei (DAPI; blue). Antibodies against (a) GM130, (b) Rab11, or (c) cathepsin D (all in red) were used. White arrows denote co-labeling of E-cadherin and cathepsin D. Cropped panels (fourth column of panels) depict boxed region of interest at higher magnification. Bars, 20 µm.





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