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Figure 4


Fig. 4. Epistatic analysis of TTC3 and CIT-K in differentiating PC12 cells. (A) PC12 cells were co-transfected with GFP-TTC3 and pDECAP control (pD-CTRL) or pDECAP CIT-K (pD-CIT), allowed to differentiate for 3 days and analyzed as in Figs 2 and 3. The image shown in the lower panel corresponds to an exemplar binucleated cells, bearing long neurites. Bar, 10 µm. (B) Quantitative analysis of differentiated cells in the experiment described in A. The first bar represents the result of a parallel control sample co-transfected with a GFP empty vector and pD-CIT. (C) PC12 cells were co-transfected with the indicated combinations of Myc-CIT-K, unrelated Myc expression plasmid (Myc-CTRL), pD-CTRL and pDECAP-TTC3 (pD-TTC3). Slides were analyzed as in A and B, with the exception that transfected cells were identified by staining with anti-Myc antibodies. Error bars represent s.d.





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