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Figure 1


Fig. 1. The apical localization of actin in ciliated airway epithelial cells during differentiation. Differentiation was induced in primary culture mTECs under ALI conditions. (A) Photomicrographs of mTEC cultures stained for filamentous actin (phalloidin, red) and for the cilia marker beta-tubulin-IV (green) on the indicated day of ALI culture. Reconstructed confocal images show z-axis view below. An apical web-like structure is seen at ALI day 3 in ciliated cells. (B) Photomicrographs obtained as in A stained for actin (phalloidin, red) and basal body marker {gamma}-tubulin (green). {gamma}-tubulin is additionally present in paired nuclear centrioles within the microtubule organizing center as seen at ALI day 2. Bars, 10 µm.





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