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Fig. 2. The effect of pharmacological inhibition of actin assembly on ciliogenesis. (A) First nine panels from left: photomicrographs showing actin (phalloidin), basal body ( ${gamma}$ -tubulin) and cilia ( $beta$ -tubulin-IV) localization in mTEC cultures at ALI day 3, obtained 1 day after treatment at ALI day 2 with vehicle, cytochalasin D or latrunculin B. Bar, 10 µm. Three panels on right: representative scanning electron micrographs (SEMs) of mTEC cultures on ALI day 4 obtained 1 day after treatment at ALI day 3. Bar, 1 µm. (B) Percentage of cells containing basal bodies localized at the apical membrane as a proportion of total cells containing basal bodies at the apical membrane or within the cytoplasm (identified by immunostaining for ${gamma}$ -tubulin). Data are the mean ± s.d.; ^*P<0.05, significance compared with vehicle. (C) The percentage of cells with apical cilia (identified by immunostaining for $beta$ -tubulin-IV) as a proportion of all cells (identified by nuclear staining with DAPI). Data are the mean ± s.d.; ^*P<0.05, significance compared with vehicle.

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