spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. NgR is expressed on plasma membrane of cultured OECs. (A) OECs were double-stained with antibodies against S-100 and NgR after treatment with Triton X-100 (a-c). OECs (d) and Schwann cells (e) were surface stained (without treatment with Triton X-100) with anti-NgR antibody. (B) Cell lysates from SCs (negative control), OECs and PC12 (positive control) cells were subjected to immunoblotting with anti-NgR (upper panel) and anti-GAPDH (lower panel). The arrowhead points to NgR (80 kDa). (C) Immunostaining for NgR on plasma membrane of OECs before (–) and after (+) treatment with PI-PLC (0.1 U/ml) at 37°C for 2 hours. (D) The supernatant (S) and membrane fractions (P) of OECs treated (+) or untreated (–) with PI-PLC (0.1 U/ml) at 37°C for 40 minutes were subjected to immunoblotting with anti-NgR (upper panel) and anti-GAPDH (lower panel). Bars, 50 µm.





Right arrow Return to article