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Figure 6


Fig. 6. Effects of OLs on the adhesion and migration of OECs. (A) OLs were identified by staining with anti-O1 and MBP(a-d). MBP-negative cell as the arrowhead points to (c,d) may be oligodendrocyte precursor cell. Nogo-A expression on OECs is revealed by immunostaining without permeabilization (e-f). (B) OECs adhere well to oligodendrocyte monolayers (OLs-MON). DiI-labelled OECs were placed over laminin or OLs-MON. The values are normalized against the laminin group. (C) Photomicrographs of OEC migration from the edge of the coverslip fragment. Coverslip fragments covered with Di-I-labbled OECs were inverted with cells facing downward onto laminin, GST, GST–Nogo-66, His, His–Nogo-66 or OLs-MON. (D) Average maximum distance traveled from the edge of the inverted fragment by OECs upon each substrate compared with laminin (100%). Migration index is the ratio of maximum migration distance traveled by OECs on a particular substrate to the maximum migration distance traveled by theses cells on laminin. (E) Distribution of the number of migrated cells occupying successive 50 µm x 1 mm areas from the edge of an inverted fragment for each substrate. **P<0.01 versus laminin. Bar, 50 µm.





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