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Fig. 7. The effect of OLs on OECs is mediated by Nogo-A. (A,B) OEC migration is not affected by oligodendrocyte-derived matrix and diffusible factors. Coverslip fragments containing OECs or OECs-DiI were inverted on laminin and cultured with culture medium (Laminin), the condition-defined medium (CM), the oligodendrocyte-conditioned medium (OLs-CM), the oligodendrocyte-derived matrix with culture medium (OLs-GM), or on a monolayer of oligodendrocytes with culture medium (OLs-MON). The migration index and distribution of the number of migrated cells were examined. Migration Index (%)=Maximum distance travelled by OECs as a percentage of the maximum distance traveled by OECs on laminin. **P<0.01 versus laminin. (C) Cos-7 cells were transfected with Nogo-A and immunostained with antibody against Nogo-A without being permeabilized with Triton X-100. (D) DiI-labelled OECs were placed over a mock-transfected Cos-7 cell monolayer (Cos-7-Mock), and a Nogo-A-transfected Cos-7 cell monolayer (Cos-7-NogoA). The adherent cells were counted and normalized against the Cos-7-Mock group. (E,F) The cell motility of OECs on a monolayer of Cos-7-NogoA was assessed using inverted coverslip migration assay. **P<0.01. (G,H) Attenuation of OEC migration by OLs-MON is NgR-dependent. OECs were pre-treated with or without PI-PLC, NgR antibody or goat IgG and applied to inverted migration assay in which OECs migrate on laminin or OLs-MON. Bars, 50 µm.
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