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Figure 1


Fig. 1. CaSEC3 is a true homologue of ScSEC3. (A) CaSEC3 can partially rescue the temperature sensitivity of Scsec3{Delta} mutants. Cells of S. cerevisiae NY2449 (SEC3), NY2448 (Scsec3{Delta}), and WYL68 (derived by transforming NY2448 with CaSEC3 under control of the ScSEC3 promoter on a CEN plasmid) were first grown overnight in synthetic medium, diluted, and streaked onto YPD plates for incubation at 22, 30 and 37°C for 2 days. (B) Casec3{Delta} cells accumulated secretory vesicles in the cytoplasm. Cells of SC5314 (SEC3) and WYL28 (sec3{Delta}) were first grown at 30°C overnight in GMM and then diluted into fresh YPD medium for further growth at 37°C for 3 hours before electron microscopy. Bar, 0.5 µm. (C) Sec3p-GFP localized to sites of cell growth. Synchronized G1 cells of WYL30 that expresses Sec3p-GFP were grown in GMM at 30°C to exponential phase (left panel). Hyphal cells (right panel) were induced in GMM supplemented with 10% serum at 37°C, and samples were collected at the indicated times for microscopy. Bar, 5 µm.





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