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Figure 2


Fig. 2. Growth defects of Casec3{Delta} cells. (A) sec3{Delta} cells exhibited temperature and rich medium sensitivity. SC5314 (SEC3) and WYL28 (sec3{Delta}) cells were grown at 30°C overnight in GMM, diluted and inoculated onto GMM or YPD agar plates for incubation at 30, 37 and 42°C for 2 days. (B) The same overnight cultures were diluted into liquid GMM or YPD medium and grown at 30°C with vigorous shaking. Growth was monitored by taking OD600 at intervals. Doubling times were calculated according to the average OD values measured during the exponential phase of growth from three independent experiments. Data are means ± s.d. of three experiments. (C) Morphological defects of sec3{Delta} cells. SC5314 (SEC3) and WYL28 (sec3{Delta}) were grown in YPD at 30 and 37°C (top) or grown in YPD containing 10% serum at 37°C for hyphal induction (bottom). Cell images were captured using differential-interference-phase contrast (DIC). Arrowheads indicate the junctions between the germ tube and the swollen daughter cell. Bars, 5 µm.





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