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Fig. 7. Genetic interactions between septin genes and SEC3. (A) Sec3p-GFP localizations in WYL30 (wild type), WYL50 (cdc10 ${Delta}$ ) and WYL55 (cdc11 ${Delta}$ ) cells under yeast growth conditions. (B) Deletion of CDC10 or CDC11 restored hyphal growth in sec3 ${Delta}$ cells. Cells of WYL55 (sec3 ${Delta}$ cdc10 ${Delta}$ ) and WYL58 (sec3 ${Delta}$ cdc11 ${Delta}$ ) were induced for hyphal growth for 4 hours, and stained with DAPI and Calcofluor White to visualize nuclei and cell walls. (C) Cdc3p-GFP exhibited normal localization in sec3 ${Delta}$ cdc10 ${Delta}$ and sec3 ${Delta}$ cdc11 ${Delta}$ hyphal cells. Cells of WYL56 and WYL59 were induced for 3 hours. (D) The sec3 ${Delta}$ cdc10 ${Delta}$ (WYL55) and sec3 ${Delta}$ cdc11 ${Delta}$ (WYL58) mutants remain temperature sensitive. Cells of SC5314 (1), sec3 ${Delta}$ (2), sec3 ${Delta}$ cdc10 ${Delta}$ (3), and sec3 ${Delta}$ cdc11 ${Delta}$ (4) were inoculated onto GMM plates and incubated at 30°C or 42°C for 2 days. (E) Genetic interactions of SEC3 with GIN4 and CLA4. Cells of WYL61 (cla4 ${Delta}$ ), WYL63 (sec3 ${Delta}$ cla4 ${Delta}$ ), WYL65 (gin4 ${Delta}$ ) and WYL67 (sec3 ${Delta}$ gin4 ${Delta}$ ) were grown in GMM containing 1 mM each of methionine and cysteine at 30°C for 3 hours (yeast growth condition); then an aliquot was inoculated into the same medium supplemented with 20% serum and incubated at 37°C for 2 hours of hyphal induction. Arrows indicate dead cells. Bars, 5 µm.

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