spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 8


Fig. 8. Cdc3p-GFP co-immunoprecipitated with Myc-Sec3p and Myc-Sec5p. Cell lysates were prepared from four strains: WYL47 (CDC3-GFP Myc-SEC3), WYL48 (CDC3-GFP Myc-SEC5), WYL49 (sec3{Delta} CDC3-GFP Myc-SEC5) and WYL38 (SEC3 CDC3-GFP). Myc-tagged proteins were precipitated by using anti-Myc beads and separated by SDS-PAGE. Cdc3p-GFP in the anti-Myc precipitates was detected by anti-GFP western blotting. WYL38 was included as a negative control and processed in the same way as other strains. 2 ml cell lysate was prepared from a 200-ml culture as described in Materials and Methods and used for co-immunoprecipitation; 30 µl of the 2-ml lysate was loaded in lanes labeled as cell lysate, and all the precipitate from the 2-ml lysate of each strain was loaded onto the gel.





Right arrow Return to article