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Fig. 6. PrP^C-Vn interaction supports axonal growth in DRG from E12.5 mouse embryos. ZrchI Prnp^+/+ (a-d) and ZrchI Prnp^0/0 (e-g) DRG were cultured on poly-L-lysine-coated coverslips (c), 200 nM Vn (a and g), 0.4 µM peptide Vn_307-320Mo (b and f), 0.4 µM peptide Vn_161-174 (d and e) and Vn plus anti-recombinant PrP^C antibody 13 µg/ml or Vn plus irrelevant IgG 13 µg/ml (h). Inset dark-field image in a shows a DRG subjected to anti-PrP^C immunohistochemistry. (h) Comparison of mean axonal growth per DRG from ZrchI Prnp^+/+ (white bars) and Prnp^0/0 mice (grey bars) for the conditions described above. ^*P<0.001 vs Pll control, Tukey's Test. (i) Comparison of mean axonal growth per DRG of at least 12 ganglia from Npu Prnp^+/+ (striped bars) and Prnp^–/– mice (black bars) for each of the following conditions: Pll, 200 nM Vn, 0.2 or 0.4 µM Vn_307-320Mo, 0.4 µM Vn_161-174, Vn plus irrelevant IgG or 0.6 µg/ml anti-PrP^C peptide 106-126. ^*P<0.001 vs Pll control, Tukey's Test. (j) The percentage of cells from ZrchI Prnp^+/+ (white bars) and ZrchI Prnp^0/0 (grey bars) dissociated DRG neurons that grow axons increased with increasing concentrations of Vn; ^*P<0.001 vs Pll control, Tukey's test.

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