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Figure 3


Fig. 3. Wrch-1 overexpression promotes focal adhesion disassembly. (A) Fluorescence micrographs of Wrch-1-overexpressing cells and control cells. HelaS3 cells were transfected with GFP-Wrch-1 or GFP and processed as described in Fig. 1. Bars, 10 µm. (B) Quantification of focal adhesions was performed as in Fig. 2D. *P<10–5, two-tailed t-test. Data are representative of two independent experiments. (C) Wrch-1 overexpression induces cell rounding. The average cell-spread area and s.e.m. were determined for ten cells for each condition as in Fig. 2C. The same micrographs as shown in Fig. 3B were used. *P<0.02, two-tailed t-test.





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